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Flavin Adenine Dinucleotide Synthesis Essay

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Flavin adenine dinucleotide

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flavin adenine dinucleotide — n a coenzyme C27H33N9O15P2 of some flavoproteins called also FAD * * * (FAD) a coenzyme composed of riboflavin 5′ phosphate (FMN) and adenosine 5′ phosphate linked by a pyrophosphate bond; it forms the prosthetic group of many… … Medical dictionary

flavin adenine dinucleotide — noun. a coenzyme C27H33N9O15P2 of certain flavoproteins (as xanthine oxidase); riboflavin 5′ adenosine diphosphate called also FAD * * * flavin adenine dinucleotide, a compound of adenosine diphosphate and flavin mononucleotide that constitutes… … Useful english dictionary

flavin adenine dinucleotide — noun Date: 1954 a coenzyme C27H33N9O15P2 of some flavoproteins … New Collegiate Dictionary

flavin adenine dinucleotide — (FAD; fla vin ad ē nĭn) An electron carrying cofactor often involved in energy production (for example, in the tricarboxylic acid cycle and the b oxidation pathway) … Dictionary of microbiology

flavin adenine dinucleotide — /ˌfleɪvən ˌædənin daɪˈnjukliətaɪd/ (say .flayvuhn .aduhneen duy nyoohkleeuhtuyd) noun a cofactor involved in some biochemical reactions catalysed by dehydrogenases, as succinate dehydrogenase in the citric acid cycle. Abbrev. FAD … Australian English dictionary

flavin adenine dinucleotide — Abbreviation: FAD A co enzyme important in various biochemical reactions. It comprises a phosphorylated vitamin B2 (riboflavin) molecule linked to AMP, and functions as a hydrogen acceptor in dehydrogenation reactions. The reduced form is… … Glossary of Biotechnology

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Flavine-Adenine Dinucleotide

CAS Registry Number: 146-14-5

CAS Name: Riboflavine 5 ¢ -(trihydrogen diphosphate) 5 ¢® 5 ¢ -ester with adenosine

Additional Names: adenosine 5 ¢ -(trihydrogen diphosphate) 5 ¢® 5 ¢ -ester with riboflavine; FAD; riboflavine 5 ¢ -adenosine diphosphate; isoalloxazine-adenine dinucleotide

Trademarks: Fademin (Chugai); Flamitajin (Teisan; Iwaki); Flanin F (Tokyo Tanabe); Flavitan

Molecular Formula: C 27 H 33 N 9 O 15 P 2

Molecular Weight: 785.55

Percent Composition: C 41.28%, H 4.23%, N 16.05%, O 30.55%, P 7.89%

Literature References: The prosthetic group of certain flavoproteins including D -amino acid oxidase, glucose oxidase, glycine oxidase, fumaric hydrogenase, histaminase, and xanthine oxidase. Isoln from yeast: Warburg et al.,Biochem. Z.297, 417 (1938). Structure and isoln from liver, kidneys, hearts, muscles: Warburg, Christian, ibid.298, 150 (1938); isoln from the mycelium of Eremothecium ashbyii: Yagi, Tada, Biochem. Prep.7, 51 (1959); Masuda et al.,US2973305 (1961 to Takeda). Synthesis: Christie et al.,J. Chem. Soc.1954, 46; Huennekens, Kilgour, J. Am. Chem. Soc.77, 6716 (1955); DeLuca, Kaplan, J. Biol. Chem.223, 569 (1956); Moffatt, Khorana, J. Am. Chem. Soc.80, 3756 (1958). Review: A. Holmgren, Experientia36 (Suppl), 149-180 (1980). Review of FAD and other flavin coenzymes: Beinert, The Enzymesvol. 2, P. D. Boyer et al., Eds. (Academic Press, New York, 2nd ed. 1960) pp 339-416; see alsovol. XIII Part B (Academic Press, New York, 3rd ed. 1975), several authors.

Derivative Type: Barium salt

Molecular Formula: C 27 H 31 BaN 9 O 15 P 2

Molecular Weight: 920.86

Percent Composition: C 35.22%, H 3.39%, Ba 14.91%, N 13.69%, O 26.06%, P 6.73%

Properties: Small yellow spheres clustered like grapes. Absorption max: 366, 445 nm. The absorption curve is practically identical with that of riboflavine. There is some stronger absorption between 450 and 510 nm resulting in aq solns which are more reddish and less green than those of riboflavine. The appearance of a strong greenish fluorescence indicates decomposition and loss of catalytic activity.

Absorption maximum: Absorption max: 366, 445 nm

Flavin adenine dinucleotide synthesis essay

Nicotinamide adenine dinucleotide phosphate

Except where noted otherwise, data are given for
materials in their standard state
(at 25 °C, 100 kPa)
Infobox disclaimer and references

Nicotinamide adenine dinucleotide phosphate (NADP +. in older notation TPN ) is used in anabolic reactions, such as fatty acid and nucleic acid synthesis, which require NADPH as a reducing agent.

NADPH is the reduced form of NADP +. and NADP + is the oxidized form of NADPH.

In chloroplasts. NADP is reduced by ferredoxin-NADP+ reductase in last step of the electron chain of the light reactions of photosynthesis. The NADPH produced is then used as reducing power for the biosynthetic reactions in the Calvin cycle of photosynthesis.

NADP ions in photosynthesis may be seen as 'dragging' hydrogen ions along with them (in the light-dependent cycles), which are used by the light-independent (Calvin) cycles to produce carbohydrates.

In animals

The oxidative phase of the pentose phosphate pathway is the major source of NADPH in cells.

NADPH provides the reducing equivalents for biosynthetic reactions and for oxidation-reduction involved in protection against the toxicity of ROS (reactive oxygen species).

NADPH is also used for anabolic pathways, such as fatty acid synthesis. cholesterol synthesis and fatty acid chain elongation.

It is the source of reducing equivalents for cytochrome 450 hydroxylation of aromatic compounds, steroids. alcohols, and drugs.

Flavin adenine dinucleotide explained

Flavin adenine dinucleotide explained

In biochemistry. flavin adenine dinucleotide (FAD ) is a redox cofactor, more specifically a prosthetic group. involved in several important reactions in metabolism. FAD can exist in three (or four: flavin-N(5)-oxide) different redox states, which are the quinone. semiquinone. and hydroquinone. FAD is converted between these states by accepting or donating electrons.

FAD, in its fully oxidized form, or quinone form, accepts two electrons and two protons to become FADH2 (hydroquinone form). The semiquinone (FADH · ) can be formed by either reduction of FAD or oxidation of FADH2 by accepting or donating one electron and one proton, respectively. See the mechanism section below for details.

A flavoprotein is a protein that contains a flavin moiety, this may be in the form of FAD or flavin mononucleotide (FMN). There are many flavoproteins besides components of the succinate dehydrogenase complex, including α-ketoglutarate dehydrogenase and a component of the pyruvate dehydrogenase complex. some examples are shown in section 6.

Flavoproteins were first discovered in 1879 by separating components of cow’s milk. They were initially called lactochrome due to their milky origin and yellow pigment. It took 50 years for the scientific community to make any substantial progress in identifying the molecules responsible for the yellow pigment. The 1930s launched the field of coenzyme research with the publication of many flavin and nicotinamide derivative structures and their obligate roles in redox catalysis. German scientists Warburg and Christian discovered a yeast derived yellow protein required for cellular respiration in 1932. Their colleague Hugo Theorell separated this yellow enzyme into apoenzyme and yellow pigment and showed that neither the enzyme alone or the pigment was capable of oxidizing NADH on their own but mixing them together would restore activity. Theorell confirmed the pigment to be riboflavins phosphate ester, flavin mononucleotide (FMN) in 1937, which was the first direct evidence for enzyme cofactors. [1] Warburg and Christian then found FAD to be a cofactor of D-amino acid oxidase through similar experiments in 1938. [2] Otto Warburg ’s work with linking nicotinamide to hydride transfers and the discovery of flavins paved the way for many scientists in the 40s and 50s to discover copious amounts of redox biochemistry and link them together in pathways such as the citric acid cycle and ATP synthesis.


Flavin adenine dinucleotide consists of two main portions: an adenine nucleotide (adenosine monophosphate ) and a flavin mononucleotide bridged together through their phosphate groups. Adenine is bound to a cyclic ribose at the 1' carbon, while phosphate is bound to the ribose at the 5' carbon to form the adenine nucledotide. Riboflavin is formed by a carbon-nitrogen (C-N) bond between a isoalloxazine and a ribitol. The phosphate group is then bound to the on the terminal ribose carbon to form a FMN. Because the bond between the isoalloxazine and the ribitol is not considered to be a glycosidic bond. the flavin mononucleotide is not truly a nucleotide. [3] This makes the dinucleotide name misleading; however, the flavin mononucleotide group is still very close to a nucleotide in its structure and chemical properties.

FAD can be reduced to FADH2 through by the addition of two H + and two e -. FADH2 can also be oxidized by the loss of one H + and one e - to form FADH. The FAD form can be recreated from another loss on one H + and one e -. FAD formation can also occur through the reduction and dehydration of flavin-N(5)-oxide. [4] Based on the oxidation state, flavins take specific colors when in aqueous solution. FAD (fully oxidized) is yellow, FADH(half reduced) is either blue or red based on the pH. and the fully reduced form is colorless. Changing the form can have a large impact on other chemical properties. For example, FAD, the fully oxidized form is subject to nucleophilic attack. the fully reduced form, FADH2 has high polarizability. while the half reduced form is unstable in aqueous solution. [5] FAD is an aromatic ring system, whereas FADH2 is not. This means that FADH2 is significantly higher in energy, without the stabilization through resonance that the aromatic structure provides. FADH2 is an energy-carrying molecule, because, once oxidized it regains aromaticity and releases the energy represented by this stabilization.

The spectroscopic properties of FAD and its variants allows for reaction monitoring by use of UV-VIS absorption and fluorescence spectroscopies. Each of the different forms of FAD have distinct absorbance spectra, making for easy observation of changes in oxidation state. [5] A major local absorbance maximum for FAD is observed at 450 nm, with an extinction coefficient of 11,300. [6] Flavins in general have fluorescent activity when unbound (proteins bound to flavin nucleic acid derivatives are called flavoprotein s). This property can be utilized when examining protein binding, observing loss of fluorescent activity when put into the bound state. [5] Oxidized flavins have high absorbances of about 450 nm, and fluoresce at about 515-520 nm.

Chemical states

In biological systems, FAD acts as an acceptor of H - and e - in its fully oxidized form, an acceptor or donor in the FADH form, and a donor in the reduced FADH2 form. The diagram below summarizes the potential changes that it can undergo.

Along with what is seen above, other reactive forms of FAD can be formed and consumed. These reactions involve the transfer of electrons and the making/breaking of chemical bonds. Through reaction mechanism s, FAD is able to contribute to chemical activities within biological systems. The following pictures depict general forms of some of the actions that FAD can be involved in.

Mechanisms 1 and 2 represent hydride gain, in which the molecule gains what amounts to be one hydride ion. Mechanisms 3 and 4 radical formation and hydride loss. Radical species contain unpaired electron atoms and are very chemically active. Hydride loss is the inverse process of the hydride gain seen before. The final two mechanisms show nucleophilic addition and a reaction using a carbon radical.


FAD plays a major role as an enzyme cofactor along with flavin mononucleotide. another molecule originating from riboflavin. [4] Bacteria, fungi and plants can produce riboflavin. but other eukaryote s, such as humans, have lost the ability to make it. [7] Therefore, humans must obtain riboflavin, also known as vitamin B2, from dietary sources. Riboflavin is generally absorbed in the small intestine and then transported to cells via carrier proteins. [7] Riboflavin kinase (EC adds a phosphate group to riboflavin to produce flavin mononucleotide, and then FAD synthetase attaches an adenine nucleotide ; both steps require ATP. [7] Bacteria generally have one bi-functional enzyme, but archaea and eukaryotes usually employ two distinct enzymes. [7] Current research indicates that distinct isoforms exist in the cytosol and mitochondria. [7] It seems that FAD is synthesized in both locations and potentially transported where needed. [5]

Flavoproteins utilize the unique and versatile structure of flavin moieties to catalyze difficult redox reactions. Since flavins have multiple redox states they can participate in processes that involve the transfer of either one or two electrons, hydrogen atoms, or hydronium ions. The N5 and C4a of the fully oxidized flavin ring are also susceptible to nucleophilic attack. [8] This wide variety of ionization and modification of the flavin moiety can be attributed to the isoalloxazine ring system and the ability of flavoproteins to drastically perturb the kinetic parameters of flavins upon binding, including flavin adenine dinucleotide (FAD).

The number of flavin-dependent protein encoded genes in the genome (the flavoproteome) is species dependent and can range from 0.1% - 3.5%, with humans having 90 flavoprotein encoded genes. [9] FAD is the more complex and abundant form of flavin and is reported to bind to 75% of the total flavoproteome [9] and 84% of human encoded flavoproteins. [10] Cellular concentrations of free or non-covalently bound flavins in a variety of cultured mammalian cell lines were reported for FAD (2.2-17.0 amol/cell) and FMN (0.46-3.4 amol/cell). [11] FAD has a more positive reduction potential than NAD+ and is a very strong oxidizing agent. The cell utilizes this in many energetically difficult oxidation reactions such as dehydrogenation of a C-C bond to an alkene. FAD-dependent proteins function in a large variety of metabolic pathways including electron transport, DNA repair, nucleotide biosynthesis, beta-oxidation of fatty acids, amino acid catabolism, as well as synthesis of other cofactors such as CoA. CoQ and heme groups. One well-known reaction is part of the citric acid cycle (also known as the TCA or Kreb's cycle); succinate dehydrogenase (complex II in the electron transport chain ) requires covalently bound FAD to catalyze the oxidation of succinate to fumarate by coupling it with the reduction of ubiquinone to ubiquinol. [5] The high-energy electrons from this oxidation are stored momentarily by reducing FAD to FADH2. FADH2 then reverts to FAD, sending its two high-energy electrons through the electron transport chain; the energy in FADH2 is enough to produce 1.5 equivalents of ATP [12] by oxidative phosphorylation. There are also redox flavoproteins that non-covalently bind to FAD like Acetyl-CoA-dehydrogenases which are involved in beta-oxidation of fatty acids and catabolism of amino acids like leucine (isovaleryl-CoA dehydrogenase ), isoleucine. (short/branched-chain acyl-CoA dehydrogenase), valine (isobutyryl-CoA dehydrogenase), and lysine (glutaryl-CoA dehydrogenase ). [13] Additional examples of FAD-dependent enzymes that regulate metabolism are glycerol-3-phosphate dehydrogenase (triglyceride synthesis) and xanthine oxidase involved in purine nucleotide catabolism. [14] There are other noncatalytic roles that FAD can play in flavoproteins such as structural roles, or involved in blue-sensitive light photoreceptors that regulate biological clocks and development, generation of light in bioluminescent bacteria. [13]


Flavoproteins have either an FMN or FAD molecule as a prosthetic group, this prosthetic group can be tightly bound or covalently linked. Only about 5-10% of flavoproteins have a covalently linked FAD, but these enzymes have stronger redox power. [5] In some instances, FAD can provide structural support for active sites or provide stabilization of intermediates during catalysis. [13] There are 90 flavoproteins in the human genome; about 84% require FAD, and around 16% require FMN, whereas 5 proteins require both to be present. [10] Flavoproteins are mainly located in the mitochondria because of their redox power. [10] Of all flavoproteins, 90% perform redox reactions and the other 10% are transferases. lyases. isomerases. ligases. [9]

Oxidation of carbon-heteroatom bonds Carbon-nitrogen

Monoamine oxidase (MAO) is an extensively studied flavoenzyme due to its biological importance with the catabolism of norepinephrine. serotonin and dopamine. MAO oxidizes primary, secondary and tertiary amines, which nonenzymatically hydrolyze from the imine to aldehyde or ketone. Even though this class of enzyme has been extensively studied, its mechanism of action is still being debated. Two mechanisms have been proposed: a radical mechanism and a nucleophilic mechanism. The radical mechanism is less generally accepted because there is currently no spectral or electron paramagnetic resonance evidence to show the presence of a radical intermediate. The nucleophilic mechanism is more favored because it is supported by site-directed mutagenesis studies which mutated two tyrosine residues that were expected to increase the nucleophilicity of the substrates. [15]


Glucose oxidase (GOX) catalyzes the oxidation of β-D-glucose to D-glucono-δ-lactone with the simultaneous reduction of enzyme-bound flavin. GOX exists as a homodimer, with each subunit binding one FAD molecule. Crystal structures show that FAD binds in a deep pocket of the enzyme near the dimer interface. Studies showed that upon replacement of FAD with 8-hydroxy-5-carba-5-deaza FAD, the stereochemistry of the reaction was determined by reacting with the re face of the flavin. During turnover, the neutral and anionic semiquinones are observed which indicates a radical mechanism. [15]


Prenylcysteine lyase (PCLase) catalyzes the cleavage of prenylcysteine (a protein modification) to form an isoprenoid aldehyde and the freed cysteine residue on the protein target. The FAD is non-covalently bound to PCLase. Not many mechanistic studies have been done looking at the reactions of the flavin, but the proposed mechanism is shown below. It is proposed that there is a hydride transfer from the C1 of the prenyl moiety to FAD that results in the reduction of the flavin to FADH2 and the formation of a carbocation that is stabilized by the neighboring sulfur atom. FADH2 then reacts with molecular oxygen to restore the oxidized enzyme. [15]


UDP-N-acetylenolpyruvylglucosamine Reductase (MurB) is an enzyme that catalyzes the NADPH-dependent reduction of enolpyruvyl-UDP-N-acetylglucosamine (substrate) to the corresponding D-lactyl compound UDP-N-acetylmuramic acid (product). MurB is a monomer and contains one FAD molecule. Before the substrate can be converted to product, NADPH must first reduce FAD. Once NADP + dissociates, the substrate can bind and the reduced flavin can reduce the product. [15]

Thiol/disulfide chemistry

Glutathione reductase (GR) catalyzes the reduction of glutathione disulfide (GSSG) to glutathione (GSH). GR requires FAD and NADPH to facilitate this reaction; first a hydride must be transferred from NADPH to FAD. The reduced flavin can then act as a nucleophile to attack the disulfide, this forms the C4a-cysteine adduct. Elimination of this adduct results in a flavin-thiolate charge-transfer complex. [15]

Electron transfer reactions

Cytochrome P-450 (CPR) enzymes contain both an FMN and FAD, as well as heme. Electrons are passed from NADPH to the FAD of CPR to the FMN and finally to cytochromes P-450. In reductive titrations, the FMN and FAD were found to both be able to exist as neutral semiquinones. The flavins are only about 4Å apart, which suggests that electron transfer is direct between them. [15]

p -Hydroxybenzoate hydroxylase (PHBH) catalyzes the oxidation of p -hydroxybenzoate (p OHB) to 3,4-dihyroxybenzoate (3,4-diOHB); FAD, NADPH and molecular oxygen are all required for this reaction. NADPH first transfers a hydride equivalent to FAD, creating FADH -. and then NADP + dissociates from the enzyme. Reduced PHBH then reacts with molecular oxygen to form the flavin hydroperoxide. The flavin hydroperoxide quickly hyrdoxylates p OHB, and then eliminates water to regenerate oxidized flavin. [15]

Chorismate synthase (CS) catalyzes the last step in the shikimate pathway —the formation of chorismate. There are two classes of CS, both of which require FMN. but are divided on their need for NADPH as a reducing agent. The proposed mechanism for CS involves radical species. The radical flavin species has not been detected spectroscopically without using a substrate analogue, which suggests that it is short-lived. However, when using a fluorinated substrate, a neutral flavin semiquinone was detected. [15]

Complex flavoenzymes

Glutamate synthase catalyzes the conversion of 2-oxoglutarate into L-glutamate with L-glutamine serving as the nitrogen source for the reaction. All glutamate synthases are iron-sulfur flavoproteins containing an iron-sulfur cluster and FMN. The three classes of glutamate synthases are categorized based on their sequences and biochemical properties. Even though there are three classes of this enzyme, it is believed that they all operate through the same mechanism, only differing by what first reduces the FMN. The enzyme produces two glutamate molecules: one by the hydrolysis of glutamine (forming glutamate and ammonia), and the second by the ammonia produced from the first reaction attacking 2-oxoglutarate, which is reduced by FMN to glutamate. [15]

Clinical signficance Flavoprotein-related diseases

Due to the importance of flavoprotein s, it is unsurprising that approximately 60% of human flavoproteins cause human disease when mutated. [10] In some cases, this is due to a decreased affinity for FAD or FMN and so excess riboflavin intake may lessen disease symptoms, such as for multiple acyl-CoA dehydrogenase deficiency. [7] In addition, riboflavin deficiency itself (and the resulting lack of FAD and FMN) can cause health issues. [7] For example, in ALS patients, there are decreased levels of FAD synthesis. [7] Both of these paths can result in a variety of symptoms, including developmental or gastrointestinal abnormalities, faulty fat break-down. anemia. neurological problems, cancer or heart disease. migraine. worsened vision and skin lesions. [7] The pharmaceutical industry therefore produces riboflavin to supplement diet in certain cases. In 2008, the global need for riboflavin was 6,000 tons per year, with production capacity of 10,000 tons. [16] This $150 to 500 million market is not only for medical applications, but is also used as a supplement to animal food in the agricultural industry and as a food colorant. [16]

Drug design

New design of anti-bacterial medications is of continuing importance in scientific research as bacterial antibiotic resistance to common antibiotics increases. A specific metabolic protein that uses FAD (Complex II ) is vital for bacterial virulence, and so targeting FAD synthesis or creating FAD analogs could be a useful area of investigation. [17] Already, scientists have determined the two structures FAD usually assumes once bound: either an extended or a butterfly conformation, in which the molecule essentially folds in half, resulting in the stacking of the adenine and isoalloxazine rings. [18] FAD imitators that are able to bind in a similar manner but do not permit protein function could be useful mechanisms of inhibiting bacterial infection. [18] Alternatively, drugs blocking FAD synthesis could achieve the same goal; this is especially intriguing because human and bacterial FAD synthesis relies on very different enzymes, meaning that a drug made to target bacterial FAD synthase would be unlikely to interfere with the human FAD synthase enzymes. [19]


Optogenetics allows control of biological events in a non-invasive manner. [20] The field has advanced in recent years with a number of new tools, including those to trigger light sensitivity, such as the Blue-Light-Utilizing FAD domains (BLUF). BLUFs encode a 100 to 140 amino acid sequence that was derived from photoreceptors in plants and bacteria. [20] Similar to other photoreceptors. the light causes structural changes in the BLUF domain that results in disruption of downstream interactions. [20] Current research investigates proteins with the appended BLUF domain and how different external factors can impact the proteins. [20]

Treatment monitoring

There are a number of molecules in the body that have native fluorescence including tryptophan, collagen. FAD, NADH and porphyrin s. [21] Scientists have taken advantage of this by using them to monitor disease progression or treatment effectiveness or aid in diagnosis. For instance, native fluorescence of a FAD and NADH is varied in normal tissue and oral submucous fibrosis. which is an early sign of invasive oral cancer. [21] Doctors therefore have been employing fluorescence to assist in diagnosis and monitor treatment as opposed to the standard biopsy. [21]

External links Notes and References
  1. Book: Hayashi. Hideyuki. vanc. B Vitamins and Folate: Chemistry, Analysis, Function and Effects. 2013. The Royal Society of Chemistry. Cambridge, UK. 978-1-84973-369-4. 7.
  2. Warburg. O. Christian. W. vanc. Isolation of the prosthetic group of the amino acid oxidase. Biochemische Zeitschrift. 1938. 298. 150–168.
  3. Book: Metzler. David E. Metzler. Carol M. Sauke. David J. vanc. Biochemistry. 2003. Harcourt, Academic Press. San Diego. 978-0-12-492541-0. 2nd.
  4. Book: Devlin. Thomas M. vanc. Textbook of Biochemistry: with Clinical Correlations. 2011. John Wiley & Sons. Hoboken, NJ. 978-0-470-28173-4. 7th.
  5. Kim HJ, Winge DR. Emerging concepts in the flavinylation of succinate dehydrogenase. Biochimica et Biophysica Acta. 1827. 5. 627–36. May 2013. 23380393. 10.1016/j.bbabio.2013.01.012.
  6. Lewis JA, Escalante-Semerena JC. The FAD-dependent tricarballylate dehydrogenase (TcuA) enzyme of Salmonella enterica converts tricarballylate into cis-aconitate. Journal of Bacteriology. 188. 15. 5479–86. Aug 2006. 16855237. 10.1128/jb.00514-06.
  7. Barile M, Giancaspero TA, Brizio C, Panebianco C, Indiveri C, Galluccio M, Vergani L, Eberini I, Gianazza E. Biosynthesis of flavin cofactors in man: implications in health and disease. Current Pharmaceutical Design. 19. 14. 2649–75. 2013. 23116402. 10.2174/1381612811319140014.
  8. Book: Monteira. Mariana. vanc. B Vitamins and Folate: Chemistry, Analysis, Function and Effects. 2013. The Royal Society of Chemistry. Cambridge, UK. 978-1-84973-369-4. 94.
  9. Macheroux P, Kappes B, Ealick SE. Flavogenomics--a genomic and structural view of flavin-dependent proteins. The FEBS Journal. 278. 15. 2625–34. Aug 2011. 21635694. 10.1111/j.1742-4658.2011.08202.x.
  10. Lienhart WD, Gudipati V, Macheroux P. The human flavoproteome. Archives of Biochemistry and Biophysics. 535. 2. 150–62. Jul 2013. 23500531. 10.1016/j.abb.2013.02.015.
  11. Hühner J, Ingles-Prieto Á, Neusüß C, Lämmerhofer M, Janovjak H. Quantification of riboflavin, flavin mononucleotide, and flavin adenine dinucleotide in mammalian model cells by CE with LED-induced fluorescence detection. Electrophoresis. 36. 4. 518–25. Feb 2015. 25488801. 10.1002/elps.201400451.
  12. Book: Stryer. Lubert. Berg. Jeremy M. Tymoczko. John L. vanc. Biochemistry. 2007. Freeman. New York. 978-0-7167-8724-2. 6th.
  13. Mansoorabadi SO, Thibodeaux CJ, Liu HW. The diverse roles of flavin coenzymes--nature's most versatile thespians. The Journal of Organic Chemistry. 72. 17. 6329–42. Aug 2007. 17580897. 10.1021/jo0703092.
  14. Web site: King. Michael W. vanc. Vitamins, Minerals, Supplements. The Medical Biochemistry Page.
  15. Fagan. Rebecca L. Palfey. Bruce A. vanc. Flavin-Dependent Enzymes. Comprehensive Natural Products II Chemistry and Biology. 2010. 7. 37–113.
  16. Abbas CA, Sibirny AA. Genetic control of biosynthesis and transport of riboflavin and flavin nucleotides and construction of robust biotechnological producers. Microbiology and Molecular Biology Reviews. 75. 2. 321–60. Jun 2011. 21646432. 10.1128/mmbr.00030-10.
  17. McNeil MB, Fineran PC. Prokaryotic assembly factors for the attachment of flavin to complex II. Biochimica et Biophysica Acta. 1827. 5. 637–47. May 2013. 22985599. 10.1016/j.bbabio.2012.09.003.
  18. Kuppuraj G, Kruise D, Yura K. Conformational behavior of flavin adenine dinucleotide: conserved stereochemistry in bound and free states. The Journal of Physical Chemistry B. 118. 47. 13486–97. Nov 2014. 25389798. 10.1021/jp507629n.
  19. Serrano A, Ferreira P, Martínez-Júlvez M, Medina M. The prokaryotic FAD synthetase family: a potential drug target. Current Pharmaceutical Design. 19. 14. 2637–48. 2013. 23116401. 10.2174/1381612811319140013.
  20. Christie JM, Gawthorne J, Young G, Fraser NJ, Roe AJ. LOV to BLUF: flavoprotein contributions to the optogenetic toolkit. Molecular Plant. 5. 3. 533–44. May 2012. 22431563. 10.1093/mp/sss020.
  21. Sivabalan S, Vedeswari CP, Jayachandran S, Koteeswaran D, Pravda C, Aruna PR, Ganesan S. In vivo native fluorescence spectroscopy and nicotinamide adinine dinucleotide/flavin adenine dinucleotide reduction and oxidation states of oral submucous fibrosis for chemopreventive drug monitoring. Journal of Biomedical Optics. 15. 1. 017010. 2010. 20210484. 10.1117/1.3324771.

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